PXD066998-1

PXD066998 is an original dataset announced via ProteomeXchange.

Title	Immunoproteasome remodeling in senescing human macrophages reveals the loss of PA28αβ capping as a hallmark of immunosenescence
Description	We established an in vitro model of blood monocyte-derived human macrophages that acquire a senescent-like phenotype upon long-term culture. Using this model, we investigated how the proteome is remodeled during senescence by analyzing cell extracts at 7 days (mature macrophages), 14 days (onset of senescence), and 28 days (fully senescent.)
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_02:11:02.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Menotta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-06 07:58:52	ID requested
⏵ 1	2025-10-06 02:11:03	announced

10.1038/s42003-025-08765-7;

Monittola F, Masini S, Montanari M, Nasoni MG, Bianchi M, De Matteis R, Ricci A, Ligi D, Luchetti F, Canonico B, Magnani M, Menotta M, Fraternale A, Crinelli R, capping as a hallmark of immunosenescence. Commun Biol, 8(1):1371(2025) [pubmed]

submitter keyword: Proteome Discoverer,Human macrophages, LFQ, MSMS, Orbitrap, DDA

Michele Menotta
contact affiliation	Department of Biomolecular Sciences University of Urbino "Carlo Bo" Campus Scientifico Enrico Mattei, Via Cà le Suore, 2 61029 Urbino (PU) ITALY
contact email	michele.menotta@uniurb.it
lab head
Michele Menotta
contact affiliation	Department of Biomolecular Sciences University of Urbino "Carlo Bo"
contact email	michele.menotta@uniurb.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066998

PRIDE project URI

• PRIDE
  1. PXD066998
     1. Label: PRIDE project
     2. Name: Immunoproteasome remodeling in senescing human macrophages reveals the loss of PA28αβ capping as a hallmark of immunosenescence