PXD066996-1

PXD066996 is an original dataset announced via ProteomeXchange.

Title	HECTD3 is a small HECT E3 ligase with unique features
Description	HECT E3 ligases regulate many cellular processes, yet how they recognise their substrates and synthesise specific types of poly-ubiquitin chains is still incompletely understood. HECTD3, a member of the “other HECT” family, is implicated in the regulation of inflammation, apoptosis, and infection and highly expressed in several cancers. These functions are largely attributed to its ligase activity and modification of diverse substrates with different types of ubiquitin chains. We present a detailed analysis of the ligase activity of HECTD3, including its ubiquitin linkage preferences, oligomeric state and substrate ubiquitination. Using cryo-EM, we provide the first full-length structures of HECTD3 in both apo and ubiquitin-loaded forms, revealing key insights into its domain organisation, including discovery of a novel fold of the N-terminal region, and mechanistic features. Some of these are shared with other HECT ligases, while others are unique to HECTD3, offering new perspectives on its catalytic mechanisms and functional diversity.
HostingRepository	PRIDE
AnnounceDate	2026-01-15
AnnouncementXML	Submission_2026-01-15_07:08:42.031.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Maslen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-06 07:51:50	ID requested
⏵ 1	2026-01-15 07:08:42	announced

Dataset with its publication pending

submitter keyword: E3 ligase, Cross-linking

Dr Katrin Rittinger
contact affiliation	Molecular Structure of Cell Signalling Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, United Kingdom
contact email	katrin.rittinger@crick.ac.uk
lab head
Sarah Maslen
contact affiliation	The Francis Crick Institute
contact email	sarah.maslen@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD066996

PRIDE project URI

• PRIDE
  1. PXD066996
     1. Label: PRIDE project
     2. Name: HECTD3 is a small HECT E3 ligase with unique features