PXD066979 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | ATPGobble: a tool to modify ATP/ADP ratio in human cells |
| Description | The ability of cells to power energy-demanding processes depends on maintaining the ATP hydrolysis reaction a billion-fold away from equilibrium. Cells respond to changes in energy state by sensing changes in ATP, ADP, AMP, and inorganic phosphate. A key barrier to a better understanding of the maintenance of energy homeostasis is a lack of tools for direct manipulation of energy state in living cells. Here, we report the development of ATPGobble–a genetically encoded tool for controlling cellular ATP hydrolysis rate. We validated ATPGobble by showing that it doubles the energy demand, decreases [ATP]/[ADP] and [ATP]/[AMP] ratios, and activates AMPK activity in human cells. We then used ATPGobble to systematically characterize the proteome and phosphoproteome changes caused by direct manipulation of the energy state. Our results establish ATPGobble as a powerful approach for dissecting the regulatory roles of energy state in human cells, opening new opportunities to study how cellular energy state governs physiology, stress responses, and disease processes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-14 |
| AnnouncementXML | Submission_2026-04-13_21:40:16.074.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alex Ekvik |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
| Instrument | Bruker Daltonics timsTOF series |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-05 20:03:44 | ID requested | |
| ⏵ 1 | 2026-04-13 21:40:16 | announced | |
Publication List
Keyword List
| submitter keyword: ATP ADP AMPK calorie restriction energy stress starvation F1 ATPase hTRET-RPE1 cells |
Contact List
| Denis V Titov |
|---|
| contact affiliation | Molecular and Cell Biology University of California, Berkeley |
| contact email | titov@berkeley.edu |
| lab head | |
| Alex Ekvik |
|---|
| contact affiliation | UC Berkeley |
| contact email | aee389@berkeley.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066979
- Label: PRIDE project
- Name: ATPGobble: a tool to modify ATP/ADP ratio in human cells
