PXD066979

PXD066979 is an original dataset announced via ProteomeXchange.

Title	ATPGobble: a tool to modify ATP/ADP ratio in human cells
Description	The ability of cells to power energy-demanding processes depends on maintaining the ATP hydrolysis reaction a billion-fold away from equilibrium. Cells respond to changes in energy state by sensing changes in ATP, ADP, AMP, and inorganic phosphate. A key barrier to a better understanding of the maintenance of energy homeostasis is a lack of tools for direct manipulation of energy state in living cells. Here, we report the development of ATPGobble–a genetically encoded tool for controlling cellular ATP hydrolysis rate. We validated ATPGobble by showing that it doubles the energy demand, decreases [ATP]/[ADP] and [ATP]/[AMP] ratios, and activates AMPK activity in human cells. We then used ATPGobble to systematically characterize the proteome and phosphoproteome changes caused by direct manipulation of the energy state. Our results establish ATPGobble as a powerful approach for dissecting the regulatory roles of energy state in human cells, opening new opportunities to study how cellular energy state governs physiology, stress responses, and disease processes.
HostingRepository	PRIDE
AnnounceDate	2026-04-14
AnnouncementXML	Submission_2026-04-13_21:40:16.074.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Ekvik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-05 20:03:44	ID requested
⏵ 1	2026-04-13 21:40:16	announced

10.1016/J.CHEMBIOL.2026.03.004;

submitter keyword: ATP ADP AMPK calorie restriction energy stress starvation F1 ATPase hTRET-RPE1 cells

Denis V Titov
contact affiliation	Molecular and Cell Biology University of California, Berkeley
contact email	titov@berkeley.edu
lab head
Alex Ekvik
contact affiliation	UC Berkeley
contact email	aee389@berkeley.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066979
     1. Label: PRIDE project
     2. Name: ATPGobble: a tool to modify ATP/ADP ratio in human cells