PXD066952-1

PXD066952 is an original dataset announced via ProteomeXchange.

Title	Metabolic dysfunction-associated steatohepatitis reduces hepatic H2S- producing enzymes altering persulfidome composition
Description	Metabolic dysfunction–associated steatohepatitis (MASH) is a progressive disease driven byobesity-related hepatic inflammation and oxidative stress. Recently, cysteine persulfidation (PSSH), a protective post-translational modification by hydrogen sulfide (H2S), was established to play a role in redox regulation. Despite the role of the liver in H2S metabolism, the function of PSSH in MASH remains underexplored. We demonstrated that H2S-producingenzymes are downregulated in both human and mouse livers with steatosis and fibrosis, resulting in a decline in global PSSH levels. Surprisingly, dimedone-switch mass spectrometry in dietary mouse models of distinct obesity-associated liver disease stages revealed dysregulated PSSH on specific proteins. In particular, increased hepatic PSSH levels of proteintyrosine phosphatases and redox regulators were found in advanced disease stages, suggesting a targeted adaptive response to oxidative stress. Overall, our findings demonstrated that impaired H2S production disrupts protective PSSH networks in MASH. However, selective PSSH preservation on redox-sensitive proteins may represent a compensatory mechanism, underscoring the therapeutic potential of persulfidation in restoring redox homeostasis during obesity-associated chronic liver disease.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-21_16:05:05.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Vignane
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-04 15:56:05	ID requested
⏵ 1	2025-09-21 16:05:06	announced

Shen TK, Vignane T, Gilglioni EH, Traini L, Kalaitsidou E, Conan P, Li A, St-Pierre-Wijckmans W, Herranz JM, Elvira B, Otero Sanchez L, Tr, é, po E, Deelman L, Wu W, Filipovic MR, Messens J, Ezeri, ņ, a D, Gurzov EN, S-producing enzymes altering persulfidome composition. Redox Biol, 86():103809(2025) [pubmed]

10.1016/j.redox.2025.103809;

submitter keyword: Persulfidome, Obesity, MASH

Milos Filipovic
contact affiliation	School of Molecular Biosciences, University of Glasgow, Glasgow, United Kingdom
contact email	Milos.Filipovic@glasgow.ac.uk
lab head
Thibaut Vignane
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS,e.V, Dortmund, Germany
contact email	thibaut.vignane@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066952

PRIDE project URI

• PRIDE
  1. PXD066952
     1. Label: PRIDE project
     2. Name: Metabolic dysfunction-associated steatohepatitis reduces hepatic H2S- producing enzymes altering persulfidome composition