PXD066932-1

PXD066932 is an original dataset announced via ProteomeXchange.

Title	Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins
Description	The mucus lining wet body surfaces forms the interphase and barrier for the microbiota and resident microbiomes. Large mucin proteins densely decorated with O-glycans make up the mucus lining to entrap, feed and shape the microbiota, and repress biofilm formation and virulence. How mucins exert these effects is poorly understood and critical is how the microbiota recognize, sense, and break down mucins. Here, we provide structural molecular evidence that a small mucin-binding module designated X409 recognizes clustered saccharide patches comprised of rows of inner monosaccharides in adjacent O-glycans. These patches are unique to mucins and binding to these provides an elegant mechanism to retain adherence to mucins despite trimming of O-glycans during microbial scavenging of monosaccharides from mucins. Realization of clustered saccharide patch-binding motifs provides a hitherto overlooked scenario of contextual glycan epitopes and impetus for discovery of new classes of glycan-binding proteins.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_16:05:22.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thapakorn Jaroentomeechai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	complex glycosylation
Instrument	autoflex; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-04 08:04:05	ID requested
⏵ 1	2025-10-19 16:05:23	announced

10.1038/s41467-025-63756-w;

Jaroentomeechai T, Veloz B, Soares CO, Goerdeler F, Grosso AS, B, ü, ll C, Miller RL, Furukawa S, Gin, é, s-Alcober I, Taleb V, Merino P, Ghirardello M, Compa, ñ, ó, n I, Coelho H, Dias JS, Vincentelli R, Henrissat B, Joshi H, Clausen H, Corzana F, Marcelo F, Hurtado-Guerrero R, Narimatsu Y, Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins. Nat Commun, 16(1):9058(2025) [pubmed]

submitter keyword: intact, MALDI

Thapakorn Jaroentomeechai
contact affiliation	University of Copenhagen
contact email	hrq262@ku.dk
lab head
Thapakorn Jaroentomeechai
contact affiliation	University of Copenhagen
contact email	hrq262@ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066932

PRIDE project URI

• PRIDE
  1. PXD066932
     1. Label: PRIDE project
     2. Name: Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins