PXD066923-1

PXD066923 is an original dataset announced via ProteomeXchange.

Title	Astrocytes modulate neuronal development by S100A6 signaling
Description	Neuronal morphogenesis relies on intercellular signaling. Astrocytes release metabolites, trophic, and guidance factors to promote neuronal maturation. In contrast, the mechanisms by which astrocytes could limit and stabilize neuronal connectivity remain less explored. Here, we find cortical astrocytes to express and release S100A6, a Ca2+-binding protein (‘calcyclin’). Simultaneously, the majority of cortical neurons expressed calcyclin-binding protein (CaCyBp), a bona fide binding partner for S100A6. In neurons, CaCyBp maintained the unfolded protein response pathway, thereby controlling proteostasis. When released, S100A6 inhibited CaCyBp-mediated signaling, thus slowing protein turnover, and, consequently, neuritogenesis. S100A6-CaCyBp signaling during gestation was sensitive to the mother’s nu tritional status, particularly eicosapentaenoic acid intake. Thus, a member of the S100 protein family acts as an astroglia-derived morphogen, whose action on neurons is modulated by environmental factors.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_01:41:10.289.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sally Shirran
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-04 04:07:32	ID requested
⏵ 1	2025-09-11 01:41:10	announced

10.1101/2023.09.05.556314;

submitter keyword: calcyclin  calcyclin-binding protein  morphogenesis  cerebral cortex intercellular commu-48 nication UPR pathway.

Tibor Harkany
contact affiliation	1Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, 13 Vienna, Austria
contact email	Tibor.Harkany@meduniwien.ac.at
lab head
Sally Shirran
contact affiliation	University of St Andrews
contact email	ss101@st-andrews.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066923
     1. Label: PRIDE project
     2. Name: Astrocytes modulate neuronal development by S100A6 signaling