PXD066917-1

PXD066917 is an original dataset announced via ProteomeXchange.

Title	Site-specific Profiling of RNA-binding Proteins Enabled by Isotopic Signature Enhanced Mass Spectrometry
Description	We developed an innovative method named “isoRIC” for profiling RNA-binding proteomes with site-specific resolution, which combined the metabolic labeling of isotopic nucleotides for photo-crosslinking of RNA-binding proteins and the real-time targeted LC-MS/MS analysis of RNA-crosslinked peptides. This method showed dramatic improvement of sensitivity in identifying RNA-crosslinked peptides with low abundance as compared to the DDA-based proteomic approaches, which enables the discovery of novel RNA-binding proteins and precise mapping of RNA-protein binding interfaces at single amino acid resolution.
HostingRepository	iProX
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-04_01:09:00.616.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yicheng Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-04 01:08:30	ID requested
⏵ 1	2025-08-04 01:09:00	announced

Dataset with its publication pending

submitter keyword: RNA binding protein, RNA crosslinked site

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Yicheng Liu
contact affiliation	Peking University
contact email	ycliu2055@outlook.com
dataset submitter

iProX dataset URI