PXD066894 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Coordination of cyanobacterial nitrate assimilation and photosynthesis by a novel PsbO-interacting protein PirN |
| Description | Nitrogen assimilation relies on photosynthetically-produced energy and reducing equivalents. Here, we identify a small protein, PsbO-interacting regulator of nitrate assimilation (PirN), as a key coordinator of photosynthesis and nitrate assimilation in Synechocystis sp. PCC 6803 (Synechocystis). PirN specifically accumulates under nitrate conditions and interacts with the photosystem II (PSII) subunit PsbO. Deletion of pirN (ΔpirN) under nitrate impairs growth, reduces PSII contents and oxygen evolution, and upregulates most nitrogen transport and assimilation proteins except the nitrate reductase NarB, mimicking nitrogen starvation response likely due to impaired nitrate reduction. PsbO expression is markedly reduced in both ΔpirN and ΔnarB. Complementation of NarB or PsbO in ΔpirN largely restores mutant growth and the expression of the other, although to different extents. These findings suggest that PirN is a nitrate-inducible regulator that hierarchically modulates NarB and PsbO expression to couple nitrate assimilation with photosynthetic activity. This coordination likely safeguards redox homeostasis when nitrate assimilation or photosynthetic electron production is perturbed. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-07_16:52:52.613.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chengcheng Huang |
| SpeciesList | scientific name: Synechocystis sp. PCC 6803 substr. GT-T; NCBI TaxID: NEWT:2930568; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-03 13:26:12 | ID requested | |
| ⏵ 1 | 2026-06-07 16:52:53 | announced | |
Publication List
| 10.1002/advs.202518047; |
| Huang C, Xiao Z, Ge H, Wang G, Dong J, Wang Y, Yang H, Wang X, Gao H, Wang Z, Yang H, Zhang Y, Huang X, Xu W, Ma W, Yang W, Wang Y, Coordination of Cyanobacterial Nitrate Assimilation and Photosynthesis by a Novel PsbO-Interacting Protein PirN. Adv Sci (Weinh), 13(29):e18047(2026) [pubmed] |
Keyword List
| submitter keyword: cyanobacteria,photosynthesis, PsbO, nitrate assimilation, PirN |
Contact List
| Wang Yingchun |
|---|
| contact affiliation | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Rd., Beijing 100101, China. |
| contact email | ycwang@genetics.ac.cn |
| lab head | |
| Chengcheng Huang |
|---|
| contact affiliation | University of Chinese Academy of Sciences |
| contact email | cc.huang@genetics.ac.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066894
- Label: PRIDE project
- Name: Coordination of cyanobacterial nitrate assimilation and photosynthesis by a novel PsbO-interacting protein PirN
