PXD066885-1

PXD066885 is an original dataset announced via ProteomeXchange.

Title	Importin 7 - HIV-1 integrase LC-MS/MS
Description	This project investigates the interaction between Importin 7 (Imp7), a nuclear transport receptor, and HIV-1 Integrase (IN), a key viral enzyme required for integration of the viral DNa to the host genome. Although Imp7 has been implicated in facilitating nuclear entry of the HIV-1 IN, the molecular details of its interaction with IN remain unclear. To characterize the physical interaction between Imp7 and IN, we applied chemical cross-linking mass spectrometry (XL-MS) using BS3 as a cross-linker. The complex was expressed, purified, and cross-linked under defined conditions, followed by tryptic digestion and LC-MS/MS analysis on an Orbitrap instrument. Five technical replicates of the same IMP7–IN complex were analyzed to ensure data robustness and reproducibility. Identified inter- and intra-protein cross-links reveal putative contact sites between Imp7 and IN, providing insight into the structural basis of their interaction. This dataset supports our broader aim of understanding host–virus interactions at the molecular level and contributes to the structural biology of nuclear import mechanisms in the context of HIV infection. All raw data files, cross-link identifications, and metadata are provided in this submission.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:14:54.482.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juana Bana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-02 09:52:06	ID requested
⏵ 1	2026-09-06 16:14:55	announced

10.1002/2211-5463.70294;

Bana J, Yariv A, Oppenheim T, Amartely H, Reichmann D, Livnah O, Importin 7 mediates the nuclear import of HIV-1 integrase via a specific interacting interface. FEBS Open Bio, ():(2026) [pubmed]

submitter keyword: HIV-1, nuclear transport

Oded Livnah
contact affiliation	Department of Biological Chemistry, Alexander Silverman Institute of Life Sciences, The Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.
contact email	oded.livnah@mail.huji.ac.il
lab head
Juana Bana
contact affiliation	The Hebrew University of Jerusalem
contact email	juana.bana@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD066885

PRIDE project URI

• PRIDE
  1. PXD066885
     1. Label: PRIDE project
     2. Name: Importin 7 - HIV-1 integrase LC-MS/MS