PXD066875-1

PXD066875 is an original dataset announced via ProteomeXchange.

Title	Multi-organ proteomic atlas of obesity regression in male mice
Description	Obesity drives systemic metabolic dysfunction, yet how the body adapts and recovers at a system-wide level remains unclear. Here we generate a multi-tissue proteomic atlas of diet-induced obesity and its regression in mice. Using a standardized, semi-automated sample preparation workflow, we quantify 12,936 unique proteins across 15 organs and 4 timepoints. We found proteomic changes to be highly tissue-dependent with a small number of proteins displaying shared changes across multiple tissues. While proteomes of most tissues revert to lean levels after weight loss, white adipose tissue retains strong phagocytic and inflammatory responses, and brain, kidney, bone, thymus, and spleen display delayed obesity-induced alterations. We map the adipose tissue-specific immune regulators using ligand-target inference and show reduced expression of proteasomal subunits in brown adipose tissue, resulting in stalled ubiquitin turnover. Finally, we make all datasets open access and create an interactive Reactome-based webtool to facilitate further community-driven discovery.
HostingRepository	PRIDE
AnnounceDate	2026-07-25
AnnouncementXML	Submission_2026-07-25_10:17:37.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felix Boel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-01 23:58:50	ID requested
⏵ 1	2026-07-25 10:17:37	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, DIA,Mouse, Multi-tissue

Blagoy Blagoev
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark
contact email	bab@bmb.sdu.dk
lab head
Felix Boel
contact affiliation	University of Southern Denmark
contact email	felixbp@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD066875

PRIDE project URI

• PRIDE
  1. PXD066875
     1. Label: PRIDE project
     2. Name: Multi-organ proteomic atlas of obesity regression in male mice