PXD066872-1

PXD066872 is an original dataset announced via ProteomeXchange.

Title	Proteome of DiFi EVs, exomeres and supermeres purified by ultracentrifugation and FPLC
Description	The cell seretome is diverse and contains extracellular secreted vesicles (EVs) as well as non-vesicular extracellular nanoparticles (NVEPs) called exomeres and supermeres, all of which carry different kinds of cell signaling cargos. We have developed new techniques to purify exomeres and supermeres by ultracentrifugation (UC) and by fast protein liquid chromatography (FPLC) from cell conditioned media and from blood plasma. We utilized a proteomics approach to compare UC and FPLC methods with standard UC and density gradient methods of isolating EVs and new methods of isolating NVEPs. We acquired LC-MS/MS data from conditioned media using standard 2D plastic and hollow fiber 3D bioreactor production from a CRC cell line, DiFi, using all these methods and growth culture conditions.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_13:36:18.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristie Lindsey Rose
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-01 16:23:40	ID requested
⏵ 1	2025-09-11 13:36:18	announced

Dataset with its publication pending

submitter keyword: supermeres, exosomes, ultracentrifugation, FPLC,proteomics, exomeres, extracellular vesicles

Kristie Lindsey Rose
contact affiliation	Vanderbilt University Proteomics Core
contact email	kristie.rose@vanderbilt.edu
lab head
Kristie Lindsey Rose
contact affiliation	Vanderbilt university
contact email	kristie.rose@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066872
     1. Label: PRIDE project
     2. Name: Proteome of DiFi EVs, exomeres and supermeres purified by ultracentrifugation and FPLC