PXD066858-1

PXD066858 is an original dataset announced via ProteomeXchange.

Title	Investigating the Interactomic Landscape of Survival Motor Neurons (SMN) and the SMN Δ7 truncated protein
Description	This protocol describes a methodology combining TurboID - a recently developed proximity biotinylation technique - with conventional epitope-tag based co-immunoprecipitation (Co-IP) to analyse protein-protein interactions (PPIs) in cell culture systems. This integrated approach allows for the targeted examination of both transient and stable protein interactors, enhancing our understanding of protein dynamics. TurboID captures transient interactions often missed by Co-IP, which captures high aCinity, stable interactors. Combination of both techniques enables direct comparison of interaction strengths, providing insights into the dynamic nature of protein interactions within cells. The rapid biotinylation capability of TurboID reduces background noise and false positives while Co- IP enriches stable interactors, together improving data quality and interpretation of the interactomic landscape. Demonstrating the eCicacy of this methodology, proteins relevant to the pathology of Spinal Muscular Atrophy were utilised to explore variations at the interactome level. The use of identical starting cell lysates for both TurboID and Co-IP minimised variability and ensured datasets were comparable, allowing for consistency and enhancing the reliability of findings regarding the nature and strength of protein interactions. This novel framework eCectively combines both innovative and classical techniques while maintaining consistency in sample handling, advancing our understanding of the intricate networks that govern cellular processes.
HostingRepository	PRIDE
AnnounceDate	2026-06-22
AnnouncementXML	Submission_2026-06-21_16:33:44.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sally Shirran
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-01 04:52:59	ID requested
⏵ 1	2026-06-21 16:33:45	announced

Beaumont B, Synowsky SA, Shirran SL, Sleeman JE, 7 truncated protein. Biotechniques, 78(1-12):207-220(2026) [pubmed]

10.1080/07366205.2026.2675476;

submitter keyword: Proximity Biotinylation, TurboID,Interactomics, SILAC, Co-Immunoprecipitation, Proteomics, Spinal Muscular Atrophy

Judith E. Sleeman
contact affiliation	School of Biology, University of St Andrews, BMS, North Haugh, St Andrews, KY16 9ST, UK.
contact email	jes14@st-andrews.ac.uk
lab head
Sally Shirran
contact affiliation	University of St Andrews
contact email	ss101@st-andrews.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD066858

PRIDE project URI

• PRIDE
  1. PXD066858
     1. Label: PRIDE project
     2. Name: Investigating the Interactomic Landscape of Survival Motor Neurons (SMN) and the SMN Δ7 truncated protein