PXD066813-1

PXD066813 is an original dataset announced via ProteomeXchange.

Title	Multiplexed Nanoparticle Protein Corona Enables Accurate and Precise Deep Plasma Proteomics
Description	The Proteograph Product Suite, a multiplexed nanoparticle (NP) protein corona-based proteomics workflow, substantially improves the depth of detection of proteins by mass spectrometry (MS) by compressing the dynamic range of protein abundances. Here, we explore the quantitative performance of the Proteograph Product Suite and its applicability to large-scale studies. Using multi-species spike-in experiments, we investigated fold-change accuracy, linearity, precision and the lower limit of quantification (LLOQ) for the Proteograph Product Suite in conjunction with multiple MS instruments. We also manufactured four distinct lots of NPs (each capable of supporting over 100,000 assays) and evaluated batch to batch reproducibility.
HostingRepository	PRIDE
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_15:18:07.354.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-31 10:19:16	ID requested
⏵ 1	2025-10-22 15:18:07	announced

10.1021/ACS.JPROTEOME.5C00729;

submitter keyword: LC-MS,Plasma proteomics, Precision, Nanoparticle, Protein corona, Quantification accuracy, Proteograph Product Suite

Asim Siddiqui
contact affiliation	Seer, Inc.
contact email	asiddiqui@seer.bio
lab head
Ting Huang
contact affiliation	Seer Inc.
contact email	thuang@seer.bio
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066813

PRIDE project URI

• PRIDE
  1. PXD066813
     1. Label: PRIDE project
     2. Name: Multiplexed Nanoparticle Protein Corona Enables Accurate and Precise Deep Plasma Proteomics