PXD066780-1

PXD066780 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics of Francisella tularensis LVS infected dendritic cells.
Description	We used immunopeptidomics to identify MHC class II peptides and antigens of the intracellular bacterial pathogen Francisella tularensis. We infected dendritic cells from mouse bone marrow with F. tularensis LVS strain or its dsbA gene mutant strain, isolated peptide-MHC-II complexes, and identified the peptides by LC-MS/MS. The identified bacterial peptides were subsequently validated as CD4 T cell epitopes in a mouse model of tularemia. We further performed proteomic analysis of LVS and LVSΔdsbA strains to determine whether there is any possible relationship between the discovered MHC-II antigens and the proteome.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_00:25:11.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marek Link
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Francisella tularensis subsp. holarctica (strain LVS); NCBI TaxID: NEWT:376619;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-30 14:35:28	ID requested
⏵ 1	2026-09-09 00:25:12	announced

Dataset with its publication pending

submitter keyword: Francisella tularensis, immunopeptidomics, antigen presentation,MHC class II

Jiri Stulik
contact affiliation	Department of Molecular Pathology and Biology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Marek Link
contact affiliation	Department of Molecular Pathology and Biology, Military Faculty of Medicine, University of Defence
contact email	marek.link@unob.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD066780

PRIDE project URI

• PRIDE
  1. PXD066780
     1. Label: PRIDE project
     2. Name: Immunopeptidomics of Francisella tularensis LVS infected dendritic cells.