PXD066774-2

PXD066774 is an original dataset announced via ProteomeXchange.

Title	CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse model
Description	Ovarian cancer remains a significant cause of cancer-related mortality, with epithelial ovarian cancer (EOC) being the most common subtype. Despite advances in treatment, the 5-year survival rate for late-stage EOC remains low due to factors such as tumor heterogeneity and an immunosuppressive tumor microenvironment (TME). This study investigates the therapeutic potential of chimeric endocrine receptor (CER) T cells engineered to express follicle stimulating hormone (FSH) in a syngeneic mouse model of EOC expressing follicle stimulating hormone receptor (ID8-FSHR). We compared two different co-stimulatory domains—CD28ζ and 4-1BBζ—in FSH-CER T cells and found that FSH-CD28ζ CER T cells exhibited enhanced cytotoxicity, proliferation, and cytokine secretion in vitro and in vivo. In the ID8-FSHR mouse model, FSH-28ζ CER T cells significantly reduced tumor burden and extended survival compared to FSH-hBBζ and control CER T cells. However, the therapeutic efficacy was compromised by T cell exhaustion, with all FSH-CER T cells expressing high levels of exhaustion markers after 7 days. In summary, incorporating a CD28ζ costimulatory domain enhances the efficacy of FSH-CER T cells, highlighting their therapeutic potential in ovarian cancer and supporting the development of strategies to mitigate immune exhaustion.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-09_08:19:29.381.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-30 09:52:30	ID requested
1	2026-04-26 19:43:43	announced
⏵ 2	2026-06-09 08:19:30	announced	2026-06-09: Updated project metadata.

10.1038/s41598-026-43225-0;

Beatty NJ, Ma M, Goala P, McSain S, Lee SB, Boucher JC, Stone ML, Kang YP, Cortes-Gomez E, Wang J, Qu J, Davila ML, CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse model. Sci Rep, 16(1):(2026) [pubmed]

submitter keyword: Astral MS, LC-MS,Mouse ascites

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences, University at Buffalo, US
contact email	junqu@buffalo.edu
lab head
Min Ma
contact affiliation	University at Buffalo
contact email	minma@buffalo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066774
     1. Label: PRIDE project
     2. Name: CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse model