PXD066763-1

PXD066763 is an original dataset announced via ProteomeXchange.

Title	Stereo-random Oligonucleotides Enable Efficient Recruitment of ADAR in vitro and in vivo
Description	We used our previously published isASO-ID protocol to identify binders for two different adenosine deaminases acting on RNA- (ADAR-) recruiting antisense oligonucleotides (ONs). The ONs differed in their phosphorothioate content and their RNA editing potency and efficiency through the recruitment of endogenous ADAR. We aimed to get a first insight into the protein interactions of this new ON class since ON toxicity is largely defined by the protein interactions. The results will help to understand class-specific mechanisms for ON toxicity and thus to design safer ON drugs.
HostingRepository	PRIDE
AnnounceDate	2025-07-31
AnnouncementXML	Submission_2025-07-31_07:32:42.602.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-30 07:04:46	ID requested
⏵ 1	2025-07-31 07:32:42	announced

Dataset with its publication pending

submitter keyword: antisense oligonucleotide, BASU, drug protein interactome, RNA Editing,proximity biotinylation, biotin ligase, SILAC, in situ biotinylation, ADAR-recruiting oligonucleotides

Thorsten Stafforst
contact affiliation	Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany Gene and RNA Therapy Center (GRTC), Faculty of Medicine, University of Tübingen, Tübingen, Germany iFIT Cluster of Excellence (EXC2180) "Image-Guided and Functionally Instructed Tumor Therapies, University of Tübingen, Tübingen, Germany
contact email	thorsten.stafforst@uni-tuebingen.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD066763

PRIDE project URI

• PRIDE
  1. PXD066763
     1. Label: PRIDE project
     2. Name: Stereo-random Oligonucleotides Enable Efficient Recruitment of ADAR in vitro and in vivo