PXD066727-1

PXD066727 is an original dataset announced via ProteomeXchange.

Title	Diurnal Rhythm of the Human Plasma Proteome
Description	This dataset supports the manuscript “Diurnal Rhythm of the Human Plasma Proteome,” which investigates how protein concentrations in human plasma fluctuate over a 24-hour period. Using high-throughput mass spectrometry, we analyzed 208 high-quality plasma samples collected every three hours from 24 healthy individuals under tightly controlled conditions. Out of 523 quantified proteins, 138 (~26%) showed significant diurnal rhythmicity. These proteins were enriched in specific tissues, particularly the liver and platelets, and were involved in key biological pathways including hemostasis, immune signaling, and metabolism. Importantly, 36 clinically relevant biomarkers displayed time-of-day-dependent variation, highlighting the need to incorporate temporal factors in diagnostic and research protocols. This dataset offers valuable insights into circadian regulation of the plasma proteome and provides a resource for researchers studying time-sensitive biomarkers and physiological processes.
HostingRepository	PRIDE
AnnounceDate	2025-09-01
AnnouncementXML	Submission_2025-08-31_16:11:17.919.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annelaura Bach Nielsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-29 10:33:20	ID requested
⏵ 1	2025-08-31 16:11:18	announced

10.1186/s12014-025-09551-7;

S J, ó, h, ö, nnuson EM, Sennels HP, J, ø, rgensen HL, Hannibal J, Yeung CC, Rasmussen C, Prus GZ, Wewer Albrechtsen NJ, Nielsen AB, Diurnal rhythm of the human plasma proteome. Clin Proteomics, 22(1):29(2025) [pubmed]

submitter keyword: Human, LC-MS, Plasma, proteome

Nicolai Jacob Wewer Albrechtsen
contact affiliation	Department of Clinical Biochemistry, Copenhagen University Hospital, Bispebjerg and Frederiksberg Hospital, Denmark
contact email	nicolai.albrechtsen@regionh.dk
lab head
Annelaura Bach Nielsen
contact affiliation	University of Copenhagen
contact email	annelaura@live.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066727

PRIDE project URI

• PRIDE
  1. PXD066727
     1. Label: PRIDE project
     2. Name: Diurnal Rhythm of the Human Plasma Proteome