PXD066724-1

PXD066724 is an original dataset announced via ProteomeXchange.

Title	Molecular Insights into Long COVID: Plasma Proteomics Reveals Oxidative Stress, Coagulation Cascade Activation, and Glycolytic Imbalance
Description	Persistent symptoms following SARS-CoV-2 infection are the hallmark of post-COVID condition (PCC), also referred to as long COVID. However, our knowledge is limited on the underlying molecular mechanisms. In this study, we performed data-independent acquisition mass spectrometry plasma proteomics (DIA-MS) to identify molecular alterations associated with PCC. DIA-MS proteomic analysis revealed a few proteins linked to oxidative stress that had altered expression. Notably, PCC samples exhibited downregulation of the antioxidant protein peroxiredoxin 6 (PRDX6) and upregulation of oxidative stress-associated proteins particularly vanin-1 (VNN1) and paraoxonase-3 (PON3). Additionally, the PCC group showed significantly higher levels of six proteins (PCSK9, CST3, C1Q, CPB2, KNG1 GAPDH), which were linked to pathways involving glycolysis, complement and coagulation cascades, and inflammation. Oxidative stress analysis confirmed that PCC samples had significantly higher levels of DNA damage (8-OHdG) than the convalescent group, whereas antioxidant markers, such as reduced and oxidized glutathione (GSH and GSSG), were significantly lower in PCC samples than in uninfected controls. Our observations point towards ongoing oxidative and inflammatory processes in PCC and suggest potential targets for biomarker development and therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2026-03-26
AnnouncementXML	Submission_2026-03-25_22:49:30.599.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean-Francois Lucier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-29 08:44:51	ID requested
⏵ 1	2026-03-25 22:49:31	announced

Dataset with its publication pending

submitter keyword: Long COVID

Post COVID-19 condition

Oxidative stress

glutathione

Plasma proteomics

Sheela Ramanathan
contact affiliation	Departments of Immunology and Cell Biology Faculty of Medicine and Health Sciences Université de Sherbrooke Sherbrooke, QC J1H 5N4 Canada
contact email	sheela.ramanathan@usherbrooke.ca
lab head
Jean-Francois Lucier
contact affiliation	Universite de Sherbrooke
contact email	jean-francois.lucier@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066724
     1. Label: PRIDE project
     2. Name: Molecular Insights into Long COVID: Plasma Proteomics Reveals Oxidative Stress, Coagulation Cascade Activation, and Glycolytic Imbalance