PXD066708-1

PXD066708 is an original dataset announced via ProteomeXchange.

Title	Acetylome Analyses Provide New Insights into the Effects of Childhood Allergic Rhinitis on Olfactory Bulb-Dependent Olfactory Impairment
Description	Background: Allergic rhinitis (AR) is a global health burden affecting 20% of the pediatric population and is characterized by nasal inflammation and frequent olfactory dysfunction. Protein acetylation, which is a frequently occurring posttranslational modification, plays pivotal roles in the regulation of olfactory bulb (OB) function. However, the role of lysine acetylation in OB pathophysiology in pediatric AR-induced olfactory dysfunction remains unexplored. Methods: In this cross-sectional study, we enrolled pediatric patients (7–18 years) diagnosed with AR and age-matched healthy controls from the Children's Hospital affiliated with the Capital Institute of Pediatrics. Olfaction was assessed using the Universal Sniff (U-Sniff) test. An ovalbumin-induced AR mouse model was established. Olfactory function was assessed via buried food and odor discrimination tests. Proteomic and acetylomic profiling of OB tissue was performed using LC‒MS/MS, with bioinformatic analyses (GO, KEGG, STRING PPI, Motif). Key acetylation enzymes were validated by immunohistochemistry. Results: A total of 162 children in the AR and healthy groups were included in this study. A decreased score on “U-Sniff” and a greater incidence of olfactory dysfunction were observed in the AR group than in the healthy group (p<0.0001). AR model mice presented severe nasal symptoms, elevated sIgE levels, and significant olfactory deficits in odor detection and discrimination. Proteomic results revealed that the expression of proteins involved in glycolysis/gluconeogenesis and propanoate metabolism was significantly dysregulated. Critically, we observed dysregulation of key acetylation-modifying enzymes, characterized by reduced expression of the acetyl-CoA generator ACSS2 and deacetylase SIRT1, alongside elevated levels of acetyltransferase P300 and deacetylase HDAC2. Global acetylome profiling revealed 3,875 lysine acetylation sites across 1,707 proteins, indicating predominant hyperacetylation (436 significantly upregulated sites on 319 proteins versus 42 downregulated sites). These acetylated proteins disrupted olfactory transduction, the synaptic vesicle cycle, the citrate cycle (TCA cycle), glycolysis/gluconeogenesis, and propanoate metabolism. Our findings provide critical insights into how nasal inflammatory processes disrupt protein acetylation networks in the olfactory bulb, advancing our understanding of the molecular basis for smell disorders in children and informing future targeted interventions.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:45:27.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruikun Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-29 01:51:27	ID requested
⏵ 1	2026-06-07 16:45:28	announced

10.1111/all.70254;

Wang R, Xu Z, Zhang Y, Wang Q, Li M, Liang J, Zhan X, Lu Y, Yuan J, Wang S, Gu Q, Acetylome Analyses Provide New Insights Into the Effects of Childhood Allergic Rhinitis on Olfactory Bulb-Dependent Olfactory Impairment. Allergy, 81(5):1891-1894(2026) [pubmed]

submitter keyword: Allergic rhinitis

Children

Olfactory dysfunction

Olfactory bulb

Lysine acetylation

Ruikun Wang
contact affiliation	Capital Institute of Pediatrics-Peking University Teaching Hospital
contact email	w13834991916@163.com
lab head
Ruikun Wang
contact affiliation	Capital Institute of Pediatrics-Peking University Teaching Hospital Beijing
contact email	w13834991916@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066708
     1. Label: PRIDE project
     2. Name: Acetylome Analyses Provide New Insights into the Effects of Childhood Allergic Rhinitis on Olfactory Bulb-Dependent Olfactory Impairment