PXD066701-1

PXD066701 is an original dataset announced via ProteomeXchange.

Title	The $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers
Description	Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To democratize access to proteomics and facilitate its integration into more high-throughput multiomic studies, we present a robust analytical framework for achieving in-depth, quantitative proteome profiling at a cost of approximately $10 per sample, termed the “$10 proteome.” Using the ThermoFisher Orbitrap Astral and Bruker timsTOF Ultra 2 mass spectrometers, we evaluated performance across sample inputs ranging from 200 pg to 100 ng and active gradient lengths from 5 to 60 minutes. Proteome coverage saturated within the low-nanogram input range, with ~8000 protein groups quantified from as little as 10 ng of input and nearly 6000 proteins from 200 pg. With already demonstrated low-cost one-pot sample preparation workflows that are appropriate for this sample input range, standardized MS acquisition settings, and high-throughput nanoLC operated at ~10 min per sample, the $10 proteome becomes feasible. This study establishes a practical, scalable, and cost-effective foundation for global proteome profiling, paving the way for routine, large-scale applications in systems biology, clinical research and beyond.
HostingRepository	PRIDE
AnnounceDate	2026-02-05
AnnouncementXML	Submission_2026-02-05_06:34:35.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Kelly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral; timsTOF Ultra 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 22:43:08	ID requested
⏵ 1	2026-02-05 06:34:36	announced

10.1101/2025.07.29.667408;

Huang S, Wang C, Lin HL, Kelly RT, The $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers. bioRxiv, ():(2025) [pubmed]

submitter keyword: limited sample input,$10 proteome

Ryan T. Kelly
contact affiliation	Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602
contact email	ryan.kelly@byu.edu
lab head
Ryan Kelly
contact affiliation	Brigham Young University
contact email	ryan.kelly@byu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066701
     1. Label: PRIDE project
     2. Name: The $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers