PXD066691-1

PXD066691 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Profiling of Exosomes Derived from Sema6A-Knockdown hPMVECs
Description	This project aims to characterize the proteomic landscape of extracellular vesicles (EVs) released by human pulmonary microvascular endothelial cells (hPMVECs) following Sema6A gene knockdown. Using lentiviral-mediated RNA interference, we generated stable Sema6A-knockdown cell lines (*sh-Sema6A*) alongside negative control cells (sh-NC). Exosomes were isolated from conditioned media of both groups via ultracentrifugation and validated for EV-specific markers (e.g., CD63, TSG101). Subsequent high-resolution tandem mass spectrometry (LC-MS/MS)-based proteomic analysis will quantitatively compare protein cargoes between *sh-Sema6A*-EVs and sh-NC-EVs. This study seeks to identify Sema6A-regulated exosomal proteins implicated in endothelial cell communication, angiogenesis, or vascular pathophysiology, providing mechanistic insights into semaphorin signaling pathways in the pulmonary vasculature.
HostingRepository	iProX
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-28_20:51:45.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renhui Huang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-28 20:51:31	ID requested
⏵ 1	2025-07-28 20:51:46	announced

Dataset with its publication pending

submitter keyword: Proteomic, Exosomes, hPMVECs

Tian Jiang
contact affiliation	Department of Thoracic Surgery, Zhongshan Hospital, Fudan University
contact email	jiangt19@fudan.edu.cn
lab head
Renhui Huang
contact affiliation	Department of Thoracic Surgery, Zhongshan Hospital, Fudan University
contact email	23211210054@m.fudan.edu.cn
dataset submitter

iProX dataset URI