⮝ Full datasets listing
PXD066617-1
PXD066617 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MAX a label-free proteomics study of the rat dentate gyrus and serum in exercise and middle age |
| Description | Label-free proteomics study of middle age and 8 weeks of voluntary wheel-running exercise in two cohorts of male Sprague-Dawley rats. The 2021_11_18 dataset contains n=80 dentate gyrus samples from n=40 rats (n=10 per group; four experimental groups). Each rat produced one dorsal and one ventral dentate section. Pooled QC samples are also included. Samples were analyzed on a Bruker timsTOF Pro in DDA mode connected to an EvoSep One running an 88-minute gradient with the 15 SPD method. Samples were sonicated in 8M Urea + 1M TEAB, reduced with DTT and alkylated with IAA, digested with Promega Seq-grade Porcine Trypsin, and desalted with ZipTips. The 2023_02_07 dataset contains serum n=40 samples from the same rats as in 2021_11_18. Pooled QC samples are also included and were used for DDA-based spectral library generation. Sera were depleted of serum albumin, transferrin, and immunoglobulin G (IgG) with a 4.6x100 mm Mouse-3 Multi Affinity Removal Column (Agilent) for high-performance liquid chromatography (HPLC). The column was connected to an Akta Purifier (GE Healthcare/Amersham Biosciences) HPLC system, running with Unicorn software (version 5.31). Sera were analyzed on a Bruker timsTOF Pro in DIA mode connected to an EvoSep One running a 44-minute gradient with the 30 SPD method. Depleted samples were solubilized in 0.5% Rapigest, reduced with DTT and alkylated with IAA, digested with Promega Seq-grade Porcine Trypsin, and desalted with ZipTips. The 2023_05_17 dataset contains n=42 dentate gyrus samples from a second cohort of rats (3 experimental groups). Each rat produced one whole dentate gyrus sample. Pooled QC samples are also included and were used for DDA-based spectral library generation. Samples were analyzed on a Bruker timsTOF Pro in DIA mode connected to an EvoSep One running an 88-minute gradient with the 15 SPD method. Samples were sonicated in 8M Urea + 1M TEAB, reduced with DTT and alkylated with IAA, digested with Promega Seq-grade Porcine Trypsin, and desalted with ZipTips. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-28 |
| AnnouncementXML | Submission_2025-10-28_06:41:10.096.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sebastian Dohm-Hansen |
| SpeciesList | scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
| ModificationList | Carbamidomethyl |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-27 04:43:52 | ID requested | |
| ⏵ 1 | 2025-10-28 06:41:10 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: aging, exercise, hippocampus, dentate gyrus, serum, LFQ, rodent, middle age, DatasetType:Proteomics |
Contact List
| Yvonne Nolan | |
|---|---|
| contact affiliation | University College Cork |
| contact email | y.nolan@ucc.ie |
| lab head | |
| Sebastian Dohm-Hansen | |
| contact affiliation | University College Cork |
| contact email | 121102711@umail.ucc.ie |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098649/ |
