PXD066561-1

PXD066561 is an original dataset announced via ProteomeXchange.

Title	Digestion-resistant proteins support the healthy metabolite profiles associated with plant-based diets
Description	Plant-based diets are known to increase healthy phenol metabolites (hippuric acid and 3-phenylpropionate) while decreasing inflammatory phenols (cresol sulfate and phenol sulfate), but there is not yet a mechanistic understanding for how plant-based diets differentially regulate these metabolites. Here, through isotope tracing studies, we show cresol and phenol sulfate primarily come from bacterial digestion of secreted protein. Through careful dietary studies, we show gut microbial access to fiber decreases secreted protein contribution and overall concentrations of these inflammatory phenols. Additionally, plant proteins increase hippuric acid and 3-phenylpropionate. Plant proteins are resistant to mammalian digestion, which increases gut microbial access to dietary protein. This increase in protein fuels production of healthy phenols regardless of the fiber or fat composition of the diet. Our data establish digestion-resistant plant protein as a modulator of microbiome metabolism. Together, fiber and digestion-resistant protein supports the healthy metabolite profiles associated with plant-based diets.
HostingRepository	PRIDE
AnnounceDate	2026-07-06
AnnouncementXML	Submission_2026-07-06_09:48:14.851.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD066561
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Felix Keber
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: gut metagenome; NCBI TaxID: NEWT:749906;
ModificationList	N-ethylmaleimide derivatized cysteine
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-24 12:14:48	ID requested
⏵ 1	2026-07-06 09:48:15	announced

10.1073/PNAS.2605226123;

10.6019/PXD066561;

submitter keyword: plant-based diets,Diet, gut microbiome, isotope, microbiome metabolism, phenylalanine

Martin Wühr
contact affiliation	Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA
contact email	wuhr@princeton.edu
lab head
Felix Keber
contact affiliation	Princeton University
contact email	fkeber@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066561
     1. Label: PRIDE project
     2. Name: Digestion-resistant proteins support the healthy metabolite profiles associated with plant-based diets