PXD066465 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Advantage of using scout-triggered multiple reaction monitoring mass spectrometry for robust quantification of host cell proteins in biotherapeutics matrices |
| Description | The European and United States Pharmacopeias require the characterization and quantification of host cell proteins (HCPs) contaminating biological products, as these proteins pose a potential risk to clinical safety and product stability. The Enzyme-linked ImmunoSorbent Assay (ELISA) is currently the reference method for quantifying total HCPs, although it does not guarantee accurate and comprehensive detection of all HCPs. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is an alternative and orthogonal technique to ELISA, offering higher sensitivity and a broader dynamic range. In this study, we demonstrate the unmatched advantages of the Scout-triggered MRM targeted acquisition method for analyzing a 240-peptide-plex assay (720 transitions) targeting 97 HCPs in various crude or purified industrial bioproduct matrices derived from Chinese hamster ovary (CHO) cell cultures. Beyond its high multiplexing capability, the main advantage of Scout-triggered MRM, compared to conventional retention time-based methods, lies in its robustness against peptide retention time shifts across different pharmaceutical products. This method allowed us to achieve a dynamic range covering six orders of magnitude, quantifying HCPs down to 2.9 ppm. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-22_16:08:13.323.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Julie Flecheux |
| SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: NEWT:10029; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q TRAP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-23 04:28:03 | ID requested | |
| ⏵ 1 | 2026-02-22 16:08:14 | announced | |
Publication List
| 10.3390/proteomes14010009; |
| Flecheux J, Bardet C, Herment L, Fortin T, Lemoine J, Scout-Triggered Multiple Reaction Monitoring Enables Robust Quantification of Host Cell Proteins Across Bioprocess Matrices. Proteomes, 14(1):(2026) [pubmed] |
Keyword List
| submitter keyword: Bioanalysis, Biopharmaceutical characterization, Host Cell Protein,Analytical biochemistry, CHO, Chinese Hamster Ovary, HCP, Antibody(s) |
Contact List
| Emmanuelle Vulliet |
|---|
| contact affiliation | Université Claude Bernard Lyon1, ISA, UMR5280, CNRS, Villeurbanne, France |
| contact email | emmanuelle.vulliet@isa-lyon.fr |
| lab head | |
| Julie Flecheux |
|---|
| contact affiliation | Université Claude Bernard Lyon1, ISA, UMR5280, CNRS, Villeurbanne, France |
| contact email | julie.flecheux@univ-lyon1.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066465
- Label: PRIDE project
- Name: Advantage of using scout-triggered multiple reaction monitoring mass spectrometry for robust quantification of host cell proteins in biotherapeutics matrices
