PXD066441-1

PXD066441 is an original dataset announced via ProteomeXchange.

Title	Algal carbon concentrating drives fatty acid biosynthesis beyond photosynthesis
Description	The global carbon cycle underpins Earth’s sustainability, with algae playing a pivotal role in biological carbon assimilation. Efficient photosynthetic carbon fixation in aquatic environments is facilitated by algal CO2-concentrating mechanisms (CCMs). Despite their importance, the coordination of inorganic carbon demands for photosynthesis with other inorganic carbon-utilizing pathways remains unexplored. Here we use the model green alga Chlamydomonas reinhardtii to demonstrate that under limited-CO2 conditions, the biotin carboxyl-carrier protein subunit of acetyl-CoA carboxylase complex—the rate-limiting enzyme in de novo fatty acid biosynthesis—forms dynamic protein condensates at the pyrenoid periphery, partially co-localizing with the putative carbonic anhydrase LCIB. Furthermore, CCM-deficient mutants exhibit compromised fatty acid biosynthesis capacity. We propose a metabolic interaction between CCM and de novo fatty acid biosynthesis, which potentially optimizes cellular carbon economy, enhances biofuel production yields and, more broadly, reveals a molecular mechanism for coordinating inorganic carbon utilization within global carbon cycle.
HostingRepository	PRIDE
AnnounceDate	2025-08-22
AnnouncementXML	Submission_2025-08-21_21:06:54.535.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqing Yang
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-22 22:21:25	ID requested
⏵ 1	2025-08-21 21:06:54	announced

Dataset with its publication pending

submitter keyword: metabolic interaction, de novo fatty acid biosynthesis, CO2-concentrating mechanisms,global carbon cycle, algae, protein condensates, pyrenoid, Chlamydomonas reinhardtii, photosynthetic carbon fixation

Xiaobo Li
contact affiliation	Westlake university
contact email	lixiaobo@westlake.edu.cn
lab head
Yuqing Yang
contact affiliation	Westlake University
contact email	yangyuqing@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD066441

PRIDE project URI

• PRIDE
  1. PXD066441
     1. Label: PRIDE project
     2. Name: Algal carbon concentrating drives fatty acid biosynthesis beyond photosynthesis