PXD066393-1

PXD066393 is an original dataset announced via ProteomeXchange.

Title	Integrated Fibre-Specific Methylome and Proteome Profiling of Human Skeletal Muscle Across Males and Females with Fibre-Type Deconvolution.
Description	We simultaneously extracted DNA and protein from >200 single fibres per participant (7 males, 5 females) to directly compare methylome and proteome profiles of Type I and Type II human skeletal muscle fibres. Using the EPICv2 array and LC-MS/MS, we identified 5,689 robust DMRs (FDR <0.001) and 338 DEPs (FDR <0.001), revealing strong inverse correlations between methylation and protein abundance in key contractile and metabolic genes. We also built a TI/TII DNA methylation reference matrix and implemented fibre-type deconvolution (MyoTYPE) for whole-muscle samples, all accessible via the MyoMETH web resource.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_17:54:41.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Steele
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-22 01:12:02	ID requested
⏵ 1	2025-10-12 17:54:42	announced

10.1186/s13395-025-00396-0;

Palmer AS, Garc, í, a-Dom, í, nguez E, Taylor MF, Garnham A, Seale K, Steele JR, Lee HC, Schittenhelm RB, Eynon N, Integrated fibre-specific methylome and proteome profiling of human skeletal muscle across males and females with fibre-type deconvolution. Skelet Muscle, 15(1):28(2025) [pubmed]

submitter keyword: Human skeletal muscle

Muscle fibres

Type I (slow-twitch)

Type II (fast-twitch)

DNA methylation

Proteome

LC-MS/MS

EPICv2 Infinium array

Deconvolution

MyoMETH

MyoTYPE.

: Prof. Nir Eynon
contact affiliation	Prof. Nir Eynon, Australian Regenerative Medicine Institute, Monash University, Clayton VIC 3800, Australia.Proteomics contact: Dr Joel R. Steele, Monash Proteomics & Metabolomics Platform, Monash Biomedicine Discovery Institute, Monash University, Clayton VIC, Australia
contact email	nir.eynon@monash.edu
lab head
Joel Steele
contact affiliation	Monash
contact email	joel.steele@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD066393

PRIDE project URI

• PRIDE
  1. PXD066393
     1. Label: PRIDE project
     2. Name: Integrated Fibre-Specific Methylome and Proteome Profiling of Human Skeletal Muscle Across Males and Females with Fibre-Type Deconvolution.