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PXD066371-2
PXD066371 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ketone Bodies-mediated Cysteine Modifications Discovered by Chemical Proteomics |
| Description | All the studies of ketone body-dependent post-translational modifications (PTMs), notably those mediated by ketone bodies, β-hydroxybutyrate (Bhb) and acetoacetate (Acac), have focused on lysine acylations. However, given the chemically diverse and reactive nature of metabolites generated, it remains unclear whether non-lysine modifications can also happen. Here, we report the synthesis of an acetoacetate-alkyne (Acac-alkyne) chemical probe that enables efficient metabolic labeling, robust fluorescent visualization, and mass spectrometry-based identification of Acac-modified proteins. By combining chemical proteomics with open-search strategy, we showed that Acac will induce previously uncharacterized cysteine modifications in mammalian cells. Notably, cysteine S-crotonation (Ccr) is validated by employing both probe-based and standard peptide-based co-elution assays. Metabolic pathway tracing further identifies BDH1 and ECHS1 as key enzymes that generate Ccr formation. Together, these findings establish ketone metabolism as a novel source of cysteine modifications and provide an alternative mechanistic pathway to explain the profound biological effects of ketone body. |
| HostingRepository | iProX |
| AnnounceDate | 2025-07-22 |
| AnnouncementXML | Submission_2026-02-02_23:11:59.488.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuanfei Zhou |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 240; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-21 20:16:17 | ID requested | |
| 1 | 2025-07-21 20:16:39 | announced | |
| ⏵ 2 | 2026-02-02 23:12:00 | announced | 2026-02-03: Add info. |
Publication List
| Zhou YF, Zhang L, Niu ZL, Wang X, Storper A, Hunt R, Zhao Y, Sharifi N, Wang ZA, Chemical Proteomics Identifies Ketogenesis-Mediated Cysteine Modifications Regulating Redox Function. Angew Chem Int Ed Engl, ():e19830(2026) [pubmed] |
Keyword List
| submitter keyword: Ketone Bodies, Cysteine Modifications, Chemical Proteomics |
Contact List
| Zhipeng Wang | |
|---|---|
| contact affiliation | Desai Sethi Urology Institute & Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine |
| contact email | zaw29@miami.edu |
| lab head | |
| Yuanfei Zhou | |
| contact affiliation | Desai Sethi Urology Institute & Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine |
| contact email | yxz2215@miami.edu |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
