⮝ Full datasets listing
PXD066364-1
PXD066364 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MapZ phosphorylation mediates EzrA recruitment to the division septum of Streptococcus pneumoniae |
| Description | Protein phosphorylation plays a critical role in the regulation of cell division and morphogenesis in many bacterial species. In Streptococcus pneumoniae, the unique serine/threonine kinase StkP phosphorylates several cell division proteins, including MapZ. MapZ dictates the positioning of the divisome, the cell division machinery, at the division septum through direct interaction with FtsZ, a conserved tubulin-like protein that forms a dynamic ring (Z-ring) required for the assembly of the divisome. While the molecular mechanisms by which MapZ localizes to the mid-cell and drives the divisome at the mid-cell are well characterized, the function of the phosphorylation of MapZ remains enigmatic. In this study, drawing from biochemical and microscopy approaches, we disentangled the role of MapZ phosphorylation in pneumococcal cell division. We provided evidence that MapZ interacts with EzrA, a cell division protein involved in the assembly and stability of the Z-ring. Using a series of interaction assays, we further demonstrated that this interaction is modulated by the phosphorylation of MapZ and that MapZ phosphorylation influences the localization and dynamics of EzrA. Last and importantly, we examined the localization of MapZ, EzrA and StkP at the single-cell level using fluorescent fusion proteins in pairwise and triple-wise combinations. This analysis demonstrated that the three proteins exhibit distinct localization patterns, thereby allowing the interaction between unphosphorylated MapZ and EzrA only at a specific late stage of the cell cycle. In conclusion, the findings support a model in which MapZ phosphorylation fine-tunes the localization and dynamics of EzrA, thereby ensuring proper cell division. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-09-04 |
| AnnouncementXML | Submission_2026-09-04_05:54:15.718.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | DELOLME |
| SpeciesList | scientific name: Streptococcus pneumoniae; NCBI TaxID: 1313; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-21 06:44:02 | ID requested | |
| ⏵ 1 | 2026-09-04 05:54:16 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Streptococcus pneumoniae, bacterial cell division, MapZ, protein phosphorylation, EzrA, DatasetType:Proteomics |
Contact List
| Christophe GRANGEASSE | |
|---|---|
| contact affiliation | MMSB-UMR5086-CNRS |
| contact email | christophe.grangeasse@cnrs.fr |
| lab head | |
| DELOLME | |
| contact affiliation | CNRS |
| contact email | frederic.delolme@ibcp.fr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098581/ |
