PXD066263-1

PXD066263 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS on mouse retina to investigate photoreceptor degeneration
Description	Intraocular anti-VEGFA injections are frequently used to counteract neovascularization in diseases such as the neovascular (wet) form of age-related macular degeneration (AMD) or proliferative diabetic retinopathy. However, in the clinical context, patients with atrophic (dry) and wet AMD in the same eye will receive anti-VEGFA injections, but there are reports that this severely promotes the development of atrophic areas in dry AMD. To study this, we used mice with a tamoxifen-dependent deletion of Vegfr2 in the eye (Vegfr2Δeye) and the light damage model to induce degeneration of photoreceptors and to mimic certain aspects of dry AMD. We examined retinal morphology and function, the degree of photoreceptor degeneration, as well as alterations of the retinal proteome. While, steady-state retinal morphology and function was not altered due to VEGF signaling deficiency, light-induced photoreceptor degeneration was drastically exacerbated in VEGF signaling deficient retinae concomitant with attenuated activation of the AKT kinase pathway. Furthermore, using single nuclei RNA sequencing data, we show that in humans Vegfr2 is predominantly expressed in macroglia cells of healthy retinae, dry and wet AMD patients, suggesting a VEGF-dependent neuroprotective crosstalk from macroglia cells to photoreceptors which might pose promising therapeutic options to attenuate photoreceptor degeneration in humans. Yet, our data also indicate that the use of anti-VEGF therapy should be carefully considered in the presence of neurodegenerative comorbidities, since deletion of VEGF signaling dramatically promoted neurodegeneration
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_19:25:28.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine von Toerne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-17 09:03:07	ID requested
⏵ 1	2026-06-14 19:25:29	announced

Bielmeier CB, Schmitt SI, Lehr V, Grundl A, Dillinger AE, J, ä, gle H, von Toerne C, Hauck SM, Erg, ü, n S, Tamm ER, Schlecht A, Neueder A, Braunger BM, VEGFR2 deletion increases susceptibility to photoreceptor degeneration through glial-neuronal interaction. Cell Death Dis, 17(1):(2026) [pubmed]

10.1038/s41419-026-08963-z;

submitter keyword: VEGFR2 deletion, photoreceptor,LC-MSMS

Barbara M. Braunger
contact affiliation	Barbara M. Braunger, Institute of Neuroanatomy, University Clinic Hamburg-Eppendorf, Hamburg, Germany
contact email	B.Braunger@uke.de
lab head
Christine von Toerne
contact affiliation	Helmholtz Zentrum München
contact email	vontoerne@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD066263

PRIDE project URI

• PRIDE
  1. PXD066263
     1. Label: PRIDE project
     2. Name: LC-MSMS on mouse retina to investigate photoreceptor degeneration