PXD066232-1

PXD066232 is an original dataset announced via ProteomeXchange.

Title	Secretome of human PBMC after ConA stimulation and incubation with A1 and A2 milk fractions of different molecular weight
Description	There is an ongoing discussion about cow’s milk and its variants primarily focusing on the A1 variant in contrast to the A2 variant and their health-related impacts. The difference between these variants lies in a single amino acid alteration at position 67 of β-casein. This alteration is presumed to make the A1 variant more susceptible to enzymatic breakdown during milk digestion, leading to an increased release of the peptide β-casomorphin-7 (BCM-7), which is discussed to be associated with various health risks. Thus, we analyzed the influence of A1 and A2 milk on Concanavalin A (ConA)-stimulated human peripheral blood mononuclear cells (PBMCs) in earlier studies. Thereby we were able to show that milk fundamentally inhibits PBMC proliferation, independent of the β-casein variant (Gard, Flad et al. 2024). This points to an anti-proliferative effect of cow´s milk on human PBMCs, regardless of the A1 or A2 β-casein variant. In the recent study, we divided milk of both ß-casein variants in a high molecular weight (>100 kDa) and a low molecular weight (<100 kDa) protein fraction, in order to narrow down the range for the causative factor of this anti-proliferative effect triggered by the milk. The data presented here are highly relevant for human medicine and dairy industry. REFERENCE: Gard, F., L. M. Flad, T. Weisser, H. Ammer and C. A. Deeg (2024). "Effects of A1 Milk, A2 Milk and the Opioid-like Peptide beta-Casomorphin-7 on the Proliferation of Human Peripheral Blood Mononuclear Cells." Biomolecules 14(6).
HostingRepository	PRIDE
AnnounceDate	2026-07-13
AnnouncementXML	Submission_2026-07-12_16:26:30.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roxane Degroote
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-16 18:58:07	ID requested
⏵ 1	2026-07-12 16:26:31	announced

Flad LM, Wei, ß, er T, Liedl J, Gard F, Petrera A, von Toerne C, Hauck SM, Deeg CA, Kleinwort KJH, Inhibitory impact of A1 and A2 cow's milk on human peripheral blood mononuclear cells identifies TOM1 as a candidate anti-inflammatory mediator. Front Immunol, 17():1728935(2026) [pubmed]

10.3389/fimmu.2026.1728935;

submitter keyword: A1/A2 milk,uantitative label-free LC-MS/MS, modulation of immune system, human PBMC

Stefanie M.
contact affiliation	Metabolomics and Proteomics Core, Helmholtz Center Munich, German Research Center for Environmental Health, D-80939 Munich, Germany
contact email	stefanie.hauck@helmholtz-munich.de
lab head
Roxane Degroote
contact affiliation	LMU Munich, Veterinary Sciences, Chair of Physiology, Lena-Christ-Straße 48, 82152 Planegg/Martinsried, Germany
contact email	r.degroote@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD066232
     1. Label: PRIDE project
     2. Name: Secretome of human PBMC after ConA stimulation and incubation with A1 and A2 milk fractions of different molecular weight