PXD066216-1

PXD066216 is an original dataset announced via ProteomeXchange.

Title	E. coli CFT073 protein expression at t= 4 hours after a temperature upshift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)
Description	We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of protein expression changes in a short term response to a temperature upshift and to characterize the suite of proteins required to respond to a 37˚C host in the first few hours. We demonstrate that a significant number of proteins are rapidly altered in expression within hours after a temperature shift. Many of these proteins are related to adhesion, biofilm formation, and immune evasion, suggesting that temperature serves as a sentinel cue to predict and prepare for niche adaptation in the host.
HostingRepository	PRIDE
AnnounceDate	2025-12-18
AnnouncementXML	Submission_2025-12-18_12:15:10.161.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD066216
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christine White-Ziegler
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-16 07:55:41	ID requested
⏵ 1	2025-12-18 12:15:10	announced

10.1128/IAI.00422-25;

10.6019/PXD066216;

submitter keyword: immune evasion, transcriptome, virulence, transcriptional regulation, stress response,temperature, RpoS, biofilm, proteome

Christine White-Ziegler
contact affiliation	Department of Biological Sciences, Smith College, Northampton, Massachusetts, USA.
contact email	cwhitezi@smith.edu
lab head
Christine White-Ziegler
contact affiliation	Smith College
contact email	cwhitezi@smith.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD066216

PRIDE project URI

• PRIDE
  1. PXD066216
     1. Label: PRIDE project
     2. Name: E. coli CFT073 protein expression at t= 4 hours after a temperature upshift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)