PXD066213 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HDX-MS analysis of a TTC7B specific nanobody to inhibit EFR3 mediated PI4KA recruitment |
| Description | Phosphatidylinositol 4 kinase III (PI4KIII/PI4KA) is an essential lipid kinase that is critical for regulating plasma membrane identity. PI4KA is primarily recruited to the plasma membrane through targeted recruitment by the proteins EFR3A and EFR3B, with these binding to the PI4KA accessory proteins TTC7 (TTC7A and TTC7B) and FAM126 (FAM126A and FAM126B). Here we characterised how both EFR3 isoforms interact with all possible TTC7 and FAM126 combinations and developed a nanobody that specifically blocks EFR3 mediated PI4KA recruitment in TTC7B containing complexes. Using a yeast display approach, we generated a nanobody that is selective for TTC7B and blocks EFR3 binding. Cryo-electron microscopy and hydrogen deuterium exchange mass spectrometry reveal that the nanobody sterically blocks EFR3 recruitment and shows an extended interface with both PI4KA and TTC7B. Overall, this work provides insight into PI4KA regulation and is a useful tool for manipulating unique complexes of PI4KA that may be valuable for future therapeutic targeting of PI4KA. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-20 |
| AnnouncementXML | Submission_2025-11-20_12:20:10.226.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | John Burke |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 120 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-16 07:18:21 | ID requested | |
| ⏵ 1 | 2025-11-20 12:20:11 | announced | |
Publication List
| Suresh S, Shaw AL, Akintola DK, Lunke M, Doerr S, Rohilla P, Balla T, Yip CK, Hansen SD, Cobb JA, Burke JE, Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA. J Biol Chem, 301(12):110886(2025) [pubmed] |
| 10.1016/j.jbc.2025.110886; |
Keyword List
| submitter keyword: PI4KA, HDX-MS, nanaobody, TTC7B |
Contact List
| John E Burke |
|---|
| contact affiliation | University of Victoria |
| contact email | jeburke@uvic.ca |
| lab head | |
| John Burke |
|---|
| contact affiliation | University of Victoria |
| contact email | jeburke@uvic.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD066213 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066213
- Label: PRIDE project
- Name: HDX-MS analysis of a TTC7B specific nanobody to inhibit EFR3 mediated PI4KA recruitment
