PXD066195-1

PXD066195 is an original dataset announced via ProteomeXchange.

Title	T-cadherin, a major adiponectin binding partner, suppresses ERK signaling in metabolic tissues
Description	T-cadherin, which is a major adiponectin binding partner, exerts various organ-protective effects. However, the specific changes in intracellular signaling that are induced by T-cadherin in metabolic tissues/cells remain unclear. We demonstrated that T-cadherin suppresses ERK signaling in both cultured cells and murine tissues. T-cadherin knockdown increased ERK phosphorylation in C2C12 myocytes and F2 endothelial cells, whereas T-cadherin overexpression suppressed ERK phosphorylation. Proteomic analysis revealed that many proteins that are downstream targets of ERK signaling were upregulated by T-cadherin knockdown in myocytes. T-cadherin knockdown in myocytes or knockout in heart or skeletal muscles altered the levels of membrane proteins that are involved in signal transduction, including IGF1R and EGFR. Ablation of T-cadherin in mice was accompanied by increased ERK signaling, leading to increased cardiac hypertrophy and decreased appropriate muscle atrophy during starvation. Thus, T-cadherin, whose protein expression is maintained by adiponectin, modulates intracellular signaling and regulates cardiac and skeletal muscle homeostasis. C2C12 myotubes were transfected with control or T-cadherin siRNA and treated with or without adiponectin (APN) (10 μg/mL) for 24 h (n=4 per group).
HostingRepository	jPOST
AnnounceDate	2026-02-19
AnnouncementXML	Submission_2026-02-18_12:45:01.489.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hirofumi Nagao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-16 00:26:49	ID requested
⏵ 1	2026-02-18 12:45:01	announced

Dataset with its publication pending

submitter keyword: C2C12 myotubes, T-cadherin, adiponectin

Hirofumi Nagao, Iichiro Shimomura
lab head
Hirofumi Nagao
contact affiliation	Graduate School of Medicine, The University of Osaka
dataset submitter

jPOST dataset URI

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