PXD066172 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3 |
| Description | DNA replication causes the dilution of parental histones along with their specific post-translational modifications. The kinetics of restoring these marks on newly incorporated histones dictate how quickly genomic domains regain their epigenetic identity after replication. H3K9me3 is restored extremely slowly; the process of reconstitution, to achieve the pre-replication levels, continues throughout the following G1. The molecular mechanisms behind this slow reconstitution are unknown. We show here that RIF1’s reassociation with heterochromatin during mitotic exit is required to set up a chromatin environment permissive for histone methyltransferases to resume H3K9me3 deposition. RIF1 facilitates the recruitment of SUV39H1, HP1α and HP1β and is required for the increased tri-methylation of H3K9 that occurs during G1. RIF1 is also indispensable for recruiting Protein Phosphatase 1 (PP1α) to heterochromatin, and the interaction between RIF1 and PP1α is essential for the maintenance of H3K9me3 levels. In addition, RIF1-PP1 complex temporally restrains the activity of Aurora kinase at heterochromatin, ensuring that phosphorylation of H3S10 does not precede replication. This creates a time- window permissive for SUV39Hs to initiate the reinstatement of H3K9me3. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-18 |
| AnnouncementXML | Submission_2026-05-18_06:13:39.503.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Moritz Voelker-Albert |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monomethylated residue; acetylated residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-15 08:13:43 | ID requested | |
| 1 | 2026-05-18 05:49:19 | announced | |
| ⏵ 2 | 2026-05-18 06:13:40 | announced | 2026-05-18: Updated project metadata. |
| 3 | 2026-05-18 06:34:51 | announced | 2026-05-18: Updated project metadata. |
Publication List
| 10.1101/2025.07.14.664777; |
| Chen N, Bandau S, Nair Pushkala Kumari RR, Prevo B, Sasaki T, Web S, di Sanzo S, Shapira D, Voelker-Albert M, Gilbert DM, Alabert C, Buonomo SBC, RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3. bioRxiv, ():(2025) [pubmed] |
Keyword List
Contact List
| Sara Buonomo |
|---|
| contact affiliation | Chair of chromatin replication and architecture, Institute of Cell Biology, University of Edinburgh, School of Biological Sciences Kings Buildings Roger Land Building, room 1.63 Alexander Crum Brown Road Edinburgh EH9 3FF, UK |
| contact email | Sara.Buonomo@ed.ac.uk |
| lab head | |
| Moritz Voelker-Albert |
|---|
| contact affiliation | MOLEQLAR Analytics GmbH |
| contact email | moritz@moleqlar.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD066172 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066172
- Label: PRIDE project
- Name: RIF1 orchestrates a multi-step restoration of post-replicative H3K9me3
