PXD066171 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chlorella vulgaris Protein Isolate Effectively Protects Lacticaseibacillus rhamnosus GG Viability During processing, Storage, and In vitro Digestion |
| Description | This study examined the protective effect of Chlorella vulgaris protein isolate (CPI) on the biological activity of Lacticaseibacillus rhamnosus GG (LGG) during lyophilisation, storage, and in vitro digestion. Prior to lyophilisation, probiotic hydrogels were either fermented to pH 4.5 (CF) or left untreated (CNT). The microstructure, physicochemical, and thermal properties of the resulting probiotic powders were analysed, along with LGG adhesion to an in vitro intestinal epithelium model. Embedment in CPI-based matrices provided effective lyoprotection, with CNT offering greater stability than CF. Elevated storage temperatures and relative humidity (RH) accelerated LGG inactivation, particularly in CF. While a sufficient proportion of LGG survived the harsh conditions during in vitro digestion, pre-fermentation had a negligible impact. Additionally, a satisfactory level of LGG adhesion to the intestinal mucus layer was observed. Overall, CPI exhibits strong stabilizing properties for LGG viability and represents a promising plant-based alternative to dairy proteins for probiotic food formulations. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-06 |
| AnnouncementXML | Submission_2026-01-06_02:39:17.285.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD066171 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Céline LECLERCQ |
| SpeciesList | scientific name: Chlorella vulgaris (Green alga); NCBI TaxID: NEWT:3077; scientific name: Lactobacillus rhamnosus GG; NCBI TaxID: NEWT:568703; |
| ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; monohydroxylated residue |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-15 07:46:44 | ID requested | |
| ⏵ 1 | 2026-01-06 02:39:17 | announced | |
Publication List
Keyword List
| submitter keyword: Probiotics, Encapsulation,Microalgae, Protein isolate, Viability, In vitro digestion |
Contact List
| Leclercq Celine |
|---|
| contact affiliation | LIST_Environmental and industrial biotechnologies Bioprocessing and formulation L-4940, BASCHARAGE LUXEMBOURG |
| contact email | celine.leclercq@list.lu |
| lab head | |
| Céline LECLERCQ |
|---|
| contact affiliation | LIST - Environmental and Industrial Biotechnologies |
| contact email | celine.leclercq@list.lu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066171
- Label: PRIDE project
- Name: Chlorella vulgaris Protein Isolate Effectively Protects Lacticaseibacillus rhamnosus GG Viability During processing, Storage, and In vitro Digestion
