PXD066167-2

PXD066167 is an original dataset announced via ProteomeXchange.

Title	Murine cytomegalovirus evolved a cell cycle regulator (m54.5) within the highly conserved viral DNA polymerase gene
Description	Systems biology approaches have revealed a surprising complexity of herpesvirus gene products. Using advanced sequencing approaches, we discovered a novel gene, m54.5, encoded within a highly conserved region of the murine cytomegalovirus (MCMV) genome. This gene produces a nuclear protein, m54.5p, abundantly expressed early during infection. We show that m54.5p interacts with host cell cycle regulators—the anaphase-promoting complex/cyclosome (APC/C) and phosphatase-6 (PP6)—to arrest cells in G1 phase and block progression into S phase. This function and underlying mechanism is reminiscent of the unrelated UL21a protein in human cytomegalovirus, illustrating how distinct viruses can evolve similar strategies to control host cell division. Despite its role in cell cycle disruption, m54.5p is not required for MCMV replication in cultured cells, suggesting redundant viral mechanisms. Our findings reveal the unexpected plasticity of herpesvirus genomes to evolve new, functional transcripts and proteins even within the most highly conserved genomic regions. Our findings thereby reshape our understanding of herpesvirus evolution and virus-host interaction.
HostingRepository	PRIDE
AnnounceDate	2026-05-19
AnnouncementXML	Submission_2026-05-19_08:49:54.409.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Zheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Murine cytomegalovirus (strain Smith); NCBI TaxID: NEWT:10367;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-15 06:00:38	ID requested
1	2026-05-16 05:29:25	announced
⏵ 2	2026-05-19 08:49:54	announced	2026-05-19: Updated project metadata.

Dataset with its publication pending

submitter keyword: None

Lars Dölken
contact affiliation	Institute for Virology, Hannover Medical School, 30625 Hannover, Germany
contact email	Doelken.Lars@mh-hannover.de
lab head
Yan Zheng
contact affiliation	Hannover Medical School
contact email	zheng.yan@mh-hannover.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD066167

PRIDE project URI

• PRIDE
  1. PXD066167
     1. Label: PRIDE project
     2. Name: Murine cytomegalovirus evolved a cell cycle regulator (m54.5) within the highly conserved viral DNA polymerase gene