⮝ Full datasets listing
PXD066164-1
PXD066164 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The yeast cell cycle proteome dynamics |
| Description | 2E8 cells were lysed in 50 ul lysis buffer (2M Guanidinium-HCl (Gua), 0.1M ammonium bicarbonate, phosphatase inhibitors (Sigma P5726P0044)) by sonication (Bioruptor, 10 cycles, 30 seconds onoff, Diagenode, Belgium) and proteins were digested as described previously (PMID27345528). Shortly, proteins were reduced with 5 mM TCEP for 10 min at 95 C and alkylated with 10 mM chloroacetamide for 30 min at 37 C. After diluting samples with 100 mM ammonium bicarbonate buffer to a final Gua concentration of 0.5M, proteins were digested by incubation with sequencing-grade modified trypsin (50, ww Promega, Madison, Wisconsin) for 12 h at 37C. After acidification using 5 TFA, peptides were desalted using C18 reverse-phase spin columns (Macrospin, Harvard Apparatus) according to the manufacturer instructions, dried under vacuum and stored at -20C until further use. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-30 |
| AnnouncementXML | Submission_2026-07-30_09:42:54.561.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alex Schmidt |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-07-15 05:09:36 | ID requested | |
| ⏵ 1 | 2026-07-30 09:42:55 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Cell cycle, TMT, Copies per cell, DatasetType:Proteomics |
Contact List
| Alexander Schmidt | |
|---|---|
| contact affiliation | Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland |
| contact email | alex.schmidt@unibas.ch |
| lab head | |
| Alex Schmidt | |
| contact affiliation | University of Basel |
| contact email | alex.schmidt@unibas.ch |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098520/ |
