PXD066116 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Distinct amyloid fibril structures formed by ALS-causing SOD1 mutants G93A and D101N |
| Description | We expressed and purified recombinant full-length wild-type human SOD1 and its G93A and D101N variants in Expi293F cells where the initiating Met was removed and the N-terminus at Ala1 was acetylated, and identified N-terminal acetylation of the mammalian cell–purified G93A , D101N , and wild-type SOD1 using mass spectrometry (MS). Analysis of the b-ions inindicated +42.01037, +42.01014, and +42.01014 Da mass shifts representing the addition of an acetyl group to Ala1, demonstrating N-terminal acetylation in the mammalian cell–purified G93A, D101N, and wild-type SOD1. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-20 |
| AnnouncementXML | Submission_2025-10-19_16:14:03.680.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Liqiang Wang |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-14 16:55:39 | ID requested | |
| ⏵ 1 | 2025-10-19 16:14:04 | announced | |
Publication List
| 10.1038/s44319-025-00557-8; |
| Zhang MY, Ma Y, Wang LQ, Xia W, Li XN, Zhao K, Chen J, Li D, Zou L, Wang Z, Liu C, Liang Y, Distinct amyloid fibril structures formed by ALS-causing SOD1 mutants G93A and D101N. EMBO Rep, 26(19):4820-4846(2025) [pubmed] |
Keyword List
| submitter keyword: Amyotrophic Lateral Sclerosis (ALS) |
| SOD1 Mutants |
| Amyloid Fibrils |
| Cryo-EM Structure |
| Toxicity. |
Contact List
| Yi Liang |
|---|
| contact affiliation | Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, China. |
| contact email | liangyi@whu.edu.cn |
| lab head | |
| Liqiang Wang |
|---|
| contact affiliation | Wuhan University |
| contact email | wangliqiang@whu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD066116
- Label: PRIDE project
- Name: Distinct amyloid fibril structures formed by ALS-causing SOD1 mutants G93A and D101N
