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PXD066102-1

PXD066102 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNSFL1C (p47) S176 phosphorylation mimic vs. nonphosphorylatable IP-MS
DescriptionHuman SH-SY5Y cells were transfected with myc-tagged p47(S176D), myc-p47(S176A), or GFP for immunoprecipitation using antibodies to myc. Gel slices were digested with trypsin for capillary-liquid chromatography-nanospray tandem mass spectrometry (Capillary-LC/MS/MS) protein identification on a Thermo Scientific orbitrap Fusion mass spectrometer equipped with an EASY-Spray Sources operated in positive ion mode. Note: the D3 sample failed to demonstrate successful p47 immunoprecipitation.
HostingRepositoryMassIVE
AnnounceDate2026-08-11
AnnouncementXMLSubmission_2026-08-11_08:41:20.602.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCharleen Chu
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-07-14 06:26:04ID requested
12026-08-11 08:41:21announced
Publication List
no publication
Keyword List
submitter keyword: NSFL1C, VCP, p47(S176D) phosphomimic, p47(S176D) nonphosphorylatable, human neuroblastoma cells, DatasetType:Proteomics
Contact List
Charleen Chu
contact affiliationUniversity of Pittsburgh School of Medicine
contact emailctc4@pitt.edu
lab head
Charleen Chu
contact affiliationUniversity of Pittsburgh
contact emailctc4@pitt.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098502/