PXD066098-1

PXD066098 is an original dataset announced via ProteomeXchange.

Title	VMAT2 dysfunction impairs vesicular dopamine sequestration, driving oxidation and α-synuclein pathology in DJ-1-linked Parkinson’s disease neurons
Description	Parkinson’s disease (PD) is characterized by the selective degeneration of dopaminergic neurons in the substantia nigra, yet its underlying molecular mechanism remains unclear. Excessive dopamine oxidation is a pathological hallmark observed in various forms of PD. Mutations in PD-associated genes, such as DJ-1, underscore the critical role of mitochondrial bioenergetics in PD pathogenesis, potentially causing mitochondrial oxidant stress and increased dopamine oxidation. Given that DJ-1 KO and LoF mutations in the gene are associated with Parkinsonism, the molecular mechanism of DJ-1's involvement is being investigated in DJ-1 KO hiPSC-derived dopaminergic neurons. DJ-1 KO neurons exhibit a number of morphological and functional defects at the synapse, which could either be driven directly by DJ-1's interaction with synaptic proteins or, indirectly, by the effects of its loss on mitochondrial metabolism. In order to validate either of these hypothesis and map the physiological interactions of DJ-1, disrupted upon its KO, we have performed IP-MS experiments using endogenous untagged DJ-1 as the bait, with a particular interest towards its synaptic interactome in dopaminergic neurons.
HostingRepository	PRIDE
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-07_01:59:45.280.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-14 00:37:14	ID requested
⏵ 1	2026-01-07 01:59:45	announced

Dataset with its publication pending

submitter keyword: synaptic vesicles, VMAT2, ATP, dopamine oxidation, clathrin, DJ-1,Parkinson’s disease

Prof. Dr. Lena F. Burbulla
contact affiliation	Metabolic Biochemistry, Biomedical Center (BMC), German Center for Neurodegenerative Diseases (DZNE), Munich Cluster for Systems Neurology (SyNergy), Faculty of Medicine, LMU Munich, Munich, Germany
contact email	Lena.Burbulla@mail03.med.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD066098

PRIDE project URI

• PRIDE
  1. PXD066098
     1. Label: PRIDE project
     2. Name: VMAT2 dysfunction impairs vesicular dopamine sequestration, driving oxidation and α-synuclein pathology in DJ-1-linked Parkinson’s disease neurons