PXD066067-1

PXD066067 is an original dataset announced via ProteomeXchange.

Title	Mechano-ID: proximity labeling of mechanically active receptors and cells
Description	A major challenge in the field of mechanobiology relates to the lack of methods that enable identification of mechanically active cells and force transmitting receptors for subsequent biochemical analysis. For example, potent T cell activation requires the transmission of biophysical forces between the T cell receptor (TCR) and its peptide-loaded major histocompatibility complex (pMHC) antigens. Therefore, methods that can chemically tag mechanically active T cells and TCRs are highly desirable, as these techniques will enable a deeper understanding of the mechanisms governing immune responses and may also have broad applications in immunotherapy. Herein, we report a technique dubbed mechano-ID, which allows for mechanically selective proximity tagging by leveraging DNA-based molecular force probes that recruit proximity tagging enzymes. We demonstrate mechano-ID tagging of T cells using microscopy and flow cytometry and this is further confirmed using western blotting of mechanically active T cell receptors.
HostingRepository	PRIDE
AnnounceDate	2025-09-26
AnnouncementXML	Submission_2025-09-26_10:04:21.361.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Gordon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-11 10:05:33	ID requested
⏵ 1	2025-09-26 10:04:21	announced

10.1021/JACS.5C05203;

submitter keyword: TCR force,T cell receptor, proximity labeling, DNA tension probes

Khalid Salaita
contact affiliation	Department of Biomedical Engineering, Georgia Institute of Technology and Emory University Department of Chemistry, Emory University
contact email	k.salaita@emory.edu
lab head
David Gordon
contact affiliation	Emory University
contact email	david.ezra.gordon@emory.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066067

PRIDE project URI

• PRIDE
  1. PXD066067
     1. Label: PRIDE project
     2. Name: Mechano-ID: proximity labeling of mechanically active receptors and cells