PXD066035-1

PXD066035 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of MSC and electrically stimulated MSC (epMSC) secretomes
Description	This study aimed to investigate the effects of electrical stimulation (ES) on the secretome of mesenchymal stem cells (MSCs). Using quantitative proteomics, we identified a total of 435 proteins secreted by MSCs and ES-treated MSCs (epMSCs), among which 418 were common between groups, with 14 and 8 unique to the epMSC and MSC groups, respectively. Differential expression analysis revealed that 42 proteins were upregulated and 49 were downregulated in the epMSC secretome. Functional enrichment analyses indicated that ES enhances the pro-angiogenic properties of MSCs by modulating pathways such as VEGFA-VEGFR2 signaling and the complement cascade. These findings provide insight into how ES alters MSC-derived secretomes to promote vascularization, which may have therapeutic relevance in regenerative medicine.
HostingRepository	PRIDE
AnnounceDate	2025-09-22
AnnouncementXML	Submission_2025-09-21_16:27:38.666.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seungjun Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-10 19:17:43	ID requested
⏵ 1	2025-09-21 16:27:39	announced

10.1002/mco2.70352;

Yi J, Lee S, Ryu C, Kim G, Kim J, Lee JY, Enhanced Angiogenic Potential of Electrically Stimulated Human Adipose-Derived Mesenchymal Stem Cells (MSCs) for Ischemic Tissue Regeneration. MedComm (2020), 6(9):e70352(2025) [pubmed]

submitter keyword: Human, stem cell

Seungjun Lee
contact affiliation	Jae Young Lee, Gwangju Institute of Science and Technology, Biomimetic materials lab
contact email	dltmdwns97@gm.gist.ac.kr
lab head
Seungjun Lee
contact affiliation	GIST
contact email	dltmdwns97@gm.gist.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD066035

PRIDE project URI

• PRIDE
  1. PXD066035
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of MSC and electrically stimulated MSC (epMSC) secretomes