PXD066008-1

PXD066008 is an original dataset announced via ProteomeXchange.

Title	Proteome characterization of single gene deletion mutants lacking zinc finger µ-proteins in Haloferax volcanii
Description	µ-proteins (≤ 70 amino acids) have important and often essential roles in all kingdoms of life, including cell motility, regulation of membrane transport and as transcription factors. In the halophilic archaeon and model system Haloferax volcanii a significant number of µ-proteins were predicted to be zinc finger proteins. Here we used mass spectrometry-based proteomics to systematically investigate the impact of single gene deletions of 19, previously uncharacterized, zinc finger µ-proteins on H. volcanii grown in glycerol media. We employed a state-of-the-art dia-PASEF acquisition strategy, detecting over 3400 proteins across the 19 deletion strains and the wild-type. The comprehensive proteome coverage enabled a systematic analysis of proteome remodeling. We found that in 11 out of the 19 mutants the proteome remodeling involved proteins annotated to play a role in cell motility, matching swarming and cell growth phenotypes we observed for these strains. Taken together, our data provide the most comprehensive proteome coverage of H. volcanii to date, and the effect of 19 different zinc-finger µ-proteins deletion strains on the proteome of this organism. The combined data provide a valuable resource for future research in the field.
HostingRepository	PRIDE
AnnounceDate	2026-02-26
AnnouncementXML	Submission_2026-02-26_10:16:30.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: NEWT:2246;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-10 05:55:43	ID requested
⏵ 1	2026-02-26 10:16:31	announced

10.1093/FEMSML/UQAG005;

submitter keyword: zinc finger proteins, diaPASEF, haloferax volcanii,small proteins

Julian Langer
contact affiliation	Max-Planck Institute of Biophysics
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD066008

PRIDE project URI

• PRIDE
  1. PXD066008
     1. Label: PRIDE project
     2. Name: Proteome characterization of single gene deletion mutants lacking zinc finger µ-proteins in Haloferax volcanii