PXD065998-1

PXD065998 is an original dataset announced via ProteomeXchange.

Title	Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination
Description	Homologous recombination (HR) is a critical repair pathway involving numerous proteins that ensure error-free repair of DNA double-strand breaks (DSBs). Dysfunction in HR components can compromise genome integrity. Despite advances, many aspects of HR remain poorly understood. Notably, even one of the earliest identified and most critical interactions, between RAD51 and BRCA2, remains incompletely characterized, mainly due to the lack of structural data. This study presents a comprehensive biophysical analysis of the RAD51–BRC repeats interaction, integrating computational and experimental approaches. Starting with an assessment of the correlation between the binding affinities of individual BRC repeats and their impact on RAD51 disassembly, our investigation extends to larger BRCA2 truncations, offering unprecedented insights into the molecular determinants of RAD51 recognition. As mutations in the BRC repeats impair RAD51 recruitment and are associated with cancer, these results provide a valuable framework for interpreting pathogenic variants and guiding targeted therapies.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_15:51:29.303.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-10 03:32:03	ID requested
⏵ 1	2026-09-09 15:51:29	announced

Dataset with its publication pending

submitter keyword: BRCA2,breast cancer, RAD51, crosslinking

Julian Langer
contact affiliation	Max-Planck Institute of Biophysics
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD065998

PRIDE project URI

• PRIDE
  1. PXD065998
     1. Label: PRIDE project
     2. Name: Breaking new ground into RAD51–BRC repeats interplay in Homologous Recombination