PXD065862-1

PXD065862 is an original dataset announced via ProteomeXchange.

Title	Remorin Nanodomains Mediate Stress Resilience Through Cell Wall-Plasma Membrane Attachments
Description	The outer cell surface of an organism is the frontline for detecting and responding to environmental stimuli. In plants, this interface consists of the plasma membrane that lies beneath the cell wall and remains associated with it through attachment sites. These wall-membrane attachments become evident upon hyperosmotic shock, when severe water loss causes the membrane to retract from the wall. Despite their long-standing observation, the molecular identity and function of these attachments remain poorly understood. Here, we identified two nanodomain-mediated mechanisms governing wall-membrane attachments: one dependent on the Cellulose Synthase Complex (CSC), whose density at the plasma membrane positively correlates with resistance to hyperosmotic stress, and the other on REMORIN (REM), which acts antagonistically to the CSC mechanism. Using proximity-labeling proteomics, we identified SHOU4/4L as REM-associated proteins that mediate this antagonism. Together, our findings reveal how membrane nanodomains pattern wall-membrane attachments to mediate plant cell resilience under water stress.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_10:03:00.723.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 11:56:18	ID requested
⏵ 1	2026-05-27 10:03:01	announced

10.1016/J.CELL.2026.05.009;

submitter keyword: AP-MS, Arabidopsis, LTI6b, wild type (Col-0),TurboID, LC-MS/MS, REM1.2

Shouling Xu
contact affiliation	Carnegie Mass Spec Facility
contact email	sxu@carnegiescience.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065862

PRIDE project URI

• PRIDE
  1. PXD065862
     1. Label: PRIDE project
     2. Name: Remorin Nanodomains Mediate Stress Resilience Through Cell Wall-Plasma Membrane Attachments