PXD065860-1

PXD065860 is an original dataset announced via ProteomeXchange.

Title	Identification of plasma membrane protein signatures in the pectin rhamnose mutant rhm1-1
Description	The outer cell surface of an organism is the frontline for detecting and responding to environmental stimuli. In plants, this interface consists of the plasma membrane that lies beneath the cell wall and remains associated with it through attachment sites. These wall-membrane attachments become evident upon hyperosmotic shock, when severe water loss causes the membrane to retract from the wall. Despite their long-standing observation, the molecular identity and function of these attachments remain poorly understood. Here, we identified a Cellulose Synthase Complex (CSC) nanodomain-mediated mechanism governing wall-membrane attachments: CSC density at the plasma membrane positively correlates with resistance to hyperosmotic stress. To identify membrane protein signatures in the pectin rhamnose mutant rhm1-1, we used a biotin ligase-based proximity labeling approach by expressing a TurboID (TbID)-YFP-LTI6b fusion protein in the wild type and the rhm1-1 genetic backgrounds.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_09:51:42.913.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 11:21:37	ID requested
⏵ 1	2026-05-27 09:51:43	announced

10.1016/J.CELL.2026.05.009;

submitter keyword: AP-MS, Arabidopsis, rhm1-1, wild type (Col-0), LTI6b,TurboID, LC-MS/MS

Shouling Xu
contact affiliation	Carnegie Mass Spec Facility
contact email	sxu@carnegiescience.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065860
     1. Label: PRIDE project
     2. Name: Identification of plasma membrane protein signatures in the pectin rhamnose mutant rhm1-1