PXD065859 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Expanding the crosslinking mass spectrometry toolbox with vinyl sulfone crosslinkers |
| Description | Crosslinking mass spectrometry (MS) is a powerful approach for probing protein structures. However, most widely used crosslinkers rely on N-hydroxysuccinimide (NHS) esters, restricting reactivity primarily to lysine residues and protein N-termini, and rendering them incompatible with many amine-containing buffers (e.g., Tris) and key biochemical cofactors (e.g., ATP). To address these limitations, we introduce two novel vinyl-sulfone-based crosslinkers. Alkyne-BVSC is an enrichable, homobifunctional crosslinker featuring an acid-cleavable alkyne handle for downstream peptide enrichment. VSD is a heterobifunctional crosslinker combining a vinyl sulfone with a diazirine moiety for UV-activated photo-crosslinking. Both reagents are synthetically accessible from inexpensive precursors and retain reactivity in amine-rich biochemical environments. We show that vinyl sulfones react with cysteine, histidine, and lysine residues, thereby expanding crosslinkable residues beyond those accessible to NHS-esters. Moreover, we develop a stub-based post-search filtering strategy that leverages the MS-cleavable nature of vinyl sulfone linkages to improve crosslink identification sensitivity. Together, these advances establish vinyl-sulfone-based crosslinkers as versatile and complementary tools for structural proteomics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-29 |
| AnnouncementXML | Submission_2025-11-29_05:53:50.223.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD065859 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Anthony Ciancone |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-07 11:20:36 | ID requested | |
| ⏵ 1 | 2025-11-29 05:53:50 | announced | |
Publication List
Keyword List
| submitter keyword: mass spectrometry, vinyl sulfone,crosslinking |
Contact List
| Francis J. O'Reilly |
|---|
| contact affiliation | NCI/NIH CSB |
| contact email | oreillyfj@nih.gov |
| lab head | |
| Anthony Ciancone |
|---|
| contact affiliation | NCI Post-Doctoral Researcher |
| contact email | cianconeam@nih.gov |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065859
- Label: PRIDE project
- Name: Expanding the crosslinking mass spectrometry toolbox with vinyl sulfone crosslinkers
