PXD065855-1

PXD065855 is an original dataset announced via ProteomeXchange.

Title	Selective ubiquitination of drug-like small molecules by the ubiquitin ligase HUWE1
Description	The small molecule BI8626 inhibits HUWE1 in vitro by competing with protein substrates for ubiquitination. To assess whether BI8626 elicits global changes in protein stabilities consistent with HUWE1 inhibition, we performed quantitative proteomic analyses of HEK293 cells upon exposure to 15 µM BI8626 for 3 different durations. Analogous global proteomic analyses were performed to compare WT and HUWE1 knock-out HEK293 cells.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_07:09:02.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 10:14:02	ID requested
⏵ 1	2026-08-18 07:09:03	announced
2	2026-08-18 08:14:30	announced	2026-08-18: Updated project metadata.

10.1038/s41467-025-63442-x;

Orth B, Pohl P, Aust F, Ji Y, Seenivasan A, Dybkov O, Liang XJ, Bock L, Leidner F, Levantovsky S, Schardey P, Sander P, Disch NJ, Trautz ML, Mizi A, Papantonis A, Lenz C, Grubm, ü, ller H, Steinchen W, Behrends C, Urlaub H, Gehringer M, Lorenz S, Selective ubiquitination of drug-like small molecules by the ubiquitin ligase HUWE1. Nat Commun, 16(1):8182(2025) [pubmed]

submitter keyword: BI8262, BI8622, HECT ligase, inhibitor, E3 enzyme

Sonja Lorenz
contact affiliation	Research Group “Ubiquitin Signaling Specificity”, Max Planck Institute for Multidisciplinary Sciences, Goettingen, Germany
contact email	sonja.lorenz@mpinat.mpg.de
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD065855

PRIDE project URI

• PRIDE
  1. PXD065855
     1. Label: PRIDE project
     2. Name: Selective ubiquitination of drug-like small molecules by the ubiquitin ligase HUWE1