PXD065853 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A novel ultra-high resolution multi-reflecting time-of-flight mass spectrometer in Y-MRT configuration for bottom-up proteomics |
| Description | The first results of using a new type of ultrahigh-resolution mass analyzer based on a planar multi-pass time-of-flight mass spectrometer with periodic reflecting lenses (Y-MRT MS) for bottom-up whole-proteome analysis are presented. The instrument achieves a resolving power of 1,000,000 for peptides across an m/z range of 200-2000, with a high repetition rate of 300 Hz (averaged to 1 Hz for enhanced dynamic range). In preliminary experiments, a single 30-minute gradient HPLC separation of 1 µg proteolytic cell digest yielded over 3700 protein groups (4500 at 5% FDR) in DirectMS1 mode (without fragmentation). Combining three technical runs increased this to 5200 protein groups at 5% FDR. Peptide ion mass measurements demonstrated an accuracy of 100-150 ppb across the whole m/z range, with a dynamic range exceeding 10⁴. In DIA mode (SWATH-DIA, 20 Th window, 30-minute gradient), 4200 protein IDs were obtained at 1% FDR. These results highlight Y-MRT mass analyzer potential as a powerful addition to existing high resolution mass spectrometers for bottom-up proteomics. Further improvements in proteome coverage and analysis time are anticipated with optimized HPLC configurations and the integration of gas-phase ion mobility separation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-05 |
| AnnouncementXML | Submission_2025-11-05_04:33:55.781.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mark Ivanov |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | time-of-flight |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-07 09:35:11 | ID requested | |
| ⏵ 1 | 2025-11-05 04:33:56 | announced | |
Publication List
Keyword List
| submitter keyword: DIA, LC-MS1,TOF |
Contact List
| Mikhail. V. Gorshkov |
|---|
| contact affiliation | V. L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, Russia |
| contact email | mike.gorshkov@gmail.com |
| lab head | |
| Mark Ivanov |
|---|
| contact affiliation | INEP CP RAS |
| contact email | markmipt@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD065853 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065853
- Label: PRIDE project
- Name: A novel ultra-high resolution multi-reflecting time-of-flight mass spectrometer in Y-MRT configuration for bottom-up proteomics
